Pre-mRNA splicing is a crucial process in eukaryotic gene expression, and dysregulation can lead to the production of abnormal proteins and cellular dysfunction, contributing to diseases like cancer, autism spectrum disorders, and ß-thalassemia. Tight regulation of splicing fidelity is essential to prevent these outcomes, with DEAD-box RNA helicases playing a key role in promoting splicing efficiency and accuracy. Specifically, the evolutionarily conserved helicase Prp5 acts as a scaffold during spliceosome assembly and helps control fidelity through its catalytic activity. Our research has revealed that the ubiquitin-like protein Hub1 binds to Prp5, enhancing its ATPase activity and promoting splicing efficiency while also allowing for some missplicing under stress conditions. By investigating the regulation of Prp5 and Hub1, we aim to deepen our understanding of their roles in splicing fidelity control, potentially uncovering targets for treating missplicing-related diseases.
Publications
Nuadthaisong J., Phetruen T., Techawisutthinan C., S. Chanarat
International journal of molecular sciences, 2022
UBL5/Hub1: An Atypical Ubiquitin-Like Protein with a Typical Role as a Stress-Responsive Regulator
S. Chanarat
International journal of molecular sciences, 2021
S. Chanarat, J. Svasti
Biochimica et biophysica acta. Molecular cell research, 2020
Emerging Roles of Ubiquitin-like Proteins in Pre-mRNA Splicing.
S. Chanarat, S. Mishra
Trends in biochemical sciences, 2018
Error-Prone Splicing Controlled by the Ubiquitin Relative Hub1.
Ramazan Karaduman, S. Chanarat, B. Pfander, S. Jentsch
Molecular cell, 2017
Splicing and beyond: the many faces of the Prp19 complex.
S. Chanarat, K. Sträßer
Biochimica et biophysica acta, 2013
Prp19C and TREX: Interacting to promote transcription elongation and mRNA export
S. Chanarat, C. Burkert-Kautzsch, D. Meinel, K. Sträßer
Transcription, 2012
S. Chanarat, M. Seizl, K. Sträßer
Genes & development, 2011
Emanuel Clausing, A. Mayer, S. Chanarat, B. Müller, S. M. Germann, P. Cramer, M. Lisby, K. Sträßer
The Journal of Biological Chemistry, 2010
Hepatitis delta antigen binds to the clamp of RNA polymerase II and affects transcriptional fidelity
Y. Yamaguchi, Takashi Mura, S. Chanarat, Sachiko Okamoto, H. Handa
Genes to cells : devoted to molecular & cellular mechanisms, 2007
T. Narita, Y. Yamaguchi, K. Yano, S. Sugimoto, S. Chanarat, T. Wada, Dong-ki Kim, J. Hasegawa, Masashi Omori, N. Inukai, M. Endoh, Tomoko Yamada, H. Handa
Molecular and Cellular Biology, 2003
