APA
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Narita, T., Yamaguchi, Y., Yano, K., Sugimoto, S., Chanarat, S., Wada, T., … Handa, H. (2003). Human Transcription Elongation Factor NELF: Identification of Novel Subunits and Reconstitution of the Functionally Active Complex. Molecular and Cellular Biology.
Chicago/Turabian
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Narita, T., Y. Yamaguchi, K. Yano, S. Sugimoto, S. Chanarat, T. Wada, Dong-ki Kim, et al. “Human Transcription Elongation Factor NELF: Identification of Novel Subunits and Reconstitution of the Functionally Active Complex.” Molecular and Cellular Biology (2003).
MLA
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Narita, T., et al. “Human Transcription Elongation Factor NELF: Identification of Novel Subunits and Reconstitution of the Functionally Active Complex.” Molecular and Cellular Biology, 2003.
BibTeX Click to copy
@article{t2003a,
title = {Human Transcription Elongation Factor NELF: Identification of Novel Subunits and Reconstitution of the Functionally Active Complex},
year = {2003},
journal = {Molecular and Cellular Biology},
author = {Narita, T. and Yamaguchi, Y. and Yano, K. and Sugimoto, S. and Chanarat, S. and Wada, T. and Kim, Dong-ki and Hasegawa, J. and Omori, Masashi and Inukai, N. and Endoh, M. and Yamada, Tomoko and Handa, H.}
}
ABSTRACT The multisubunit transcription elongation factor NELF (for negative elongation factor) acts together with DRB (5,6-dichloro-1-β-d-ribofuranosylbenzimidazole) sensitivity-inducing factor (DSIF)/human Spt4-Spt5 to cause transcriptional pausing of RNA polymerase II (RNAPII). NELF activity is associated with five polypeptides, A to E. NELF-A has sequence similarity to hepatitis delta antigen (HDAg), the viral protein that binds to and activates RNAPII, whereas NELF-E is an RNA-binding protein whose RNA-binding activity is critical for NELF function. To understand the interactions of DSIF, NELF, and RNAPII at a molecular level, we identified the B, C, and D proteins of human NELF. NELF-B is identical to COBRA1, recently reported to associate with the product of breast cancer susceptibility gene BRCA1. NELF-C and NELF-D are highly related or identical to the protein called TH1, of unknown function. NELF-B and NELF-C or NELF-D are integral subunits that bring NELF-A and NELF-E together, and coexpression of these four proteins in insect cells resulted in the reconstitution of functionally active NELF. Detailed analyses using mutated recombinant complexes indicated that the small region of NELF-A with similarity to HDAg is critical for RNAPII binding and for transcriptional pausing. This study defines several important protein-protein interactions and opens the way for understanding the mechanism of DSIF- and NELF-induced transcriptional pausing.
